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November 9, 2018

Prolotherapy and Prolozone


Prolotherapy is the founding father of regenerative medicine and is named after its ability to encourage “proliferation”, as in the growth and repair of  ligament and joint and musculoskeletal tissues, encouraging healing while preventing further deterioration. 

Prolotherapy involves the use of natural materials that act as proliferants that are injected directly into exact anatomical locations, turning on the growth and repair response of targeted musculoskeletal tissues.  These proliferant materials act as growth and repair signalers to the chronically injured and painful musculoskeletal tissue.

Over the years a variety of proliferants have been used.  The most common, safe and inexpensive proliferant, and the one we use at the Hawaii Island Center for Regenerative Medicine, is an injection of high concentration dextrose (sugar water) solution. A variety of vitamins and nutrients are added to the dextrose solution in order to increase efficacy.   The presence of the solution in the affected musculoskeletal area causes a mild and controlled irritation to the targeted tissue to which eventually leads to an increase in blood flow and nutrients and an up regulation of the body’s natural healing response.


Ozone is a natural medical therapy that works though the creation of oxygenated signaling molecules and metabolic molecules, resulting in up regulation of metabolism and modulation of key regulatory processes in the body.  For a full discussion of ozone in medicine see our discussion on the page. 

At the Hawaii Island Center for Regenerative Medicine, the basic prolotherapy mixture is usually accompanied by an injection of ozone gas, in a technique called Prolozone, which was termed by Dr. Frank Scallenberger MD, HMD, ABAAM.  Dr. Lawinski received formal prolozone training with Frank Schallenberger in 2013. Over the years, Dr. Shallenberger and hundreds of other ozone doctors have found that the use of ozone in the proliferant mixture causes the joints and ligaments to heal more rapidly than in traditional prolotherapy.  We theorize that ozone combines with signaling molecules in the body, enhancing those signaling molecule’s ability to control growth and repair. Ozonated signaling molecules also act as regulators of the immune system, which results in less inflammation within chronically inflamed areas. The results are consistently better healing of damaged tissue with the resultant increase in function and reduction of pain. 

A short list of the conditions we treat with prolotherapy and prolozone at Hawaii Island Stem Cell Therapy and Regenerative Medicine includes:

  • Chronic Myofascial Pain

  • Back & Neck Pain

  • Osteoarthritis

  • Sports Injury

  • Rheumatoid Arthritis

  • Neuropathy

  • Chronic Ligament Pain

  • Ligament Inflammation & Laxity

  • Rotator Cuff Injury

  • Knee Arthritis & Meniscal Tears

  • Plantar Fasciitis

  • Ankle Sprains/Arthritis

  • Hip Pain

  • Chronic Strain

  • Chronic Tendonitis

  • Carpal Tunnel

Please contact us or schedule an appointment to see if regenerative musculoskeletal medicine at Hawaii Island Stem Cell Therapy and Regenerative Medicine is right for you.


Reeves. MD ( 2014) Summary of Completed Research in Prolotherapy and Bioregenerative injection, University of Kansas.

Shallenberger, Frank. Principles and Applications of ozone therapy - a practical guideline for physicians. 2011. Shallenberger, M.D., HMD, ABAAM